芯片瞬间等速电泳-筛分电泳偶联分析精确计算DNA长度

建立了基于相对迁移时间比例的方法,依据待侧DNA片段相对于上位及下位内标的迁移时间比例进行长度预测。实验结果显示,DNA片段的相对迁移时间比例在不同分析条件下具有良好的重现性。通过建立相对迁移时间比例相对于DNA片段长度的对应关系公式,实现了芯片瞬间等速电泳条件下DNA片段长度的准确预测。实际样品分析证实这种基于相对迁移时间比例的计算方法简单可靠,适合于芯片tITP-CGE分析中DNA长度的精确判定。     

几种标准蛋白质的毛细管区带电泳的分离及其迁移行为

本文采用毛细管区带电泳分离了几种标准蛋白,研究了不同的清洗方式,不同ＰＨ的缓冲溶液等对迁移行为及分离的影响,并同时考察了迁移时间和相对迁移时间与操作电压的关系。     

Determination of caffeine and its metabolites in urine by capillary electrophoresis-mass spectrometry

The caffeine content of foods and beverages varies considerably, interfering with our ability to obtain valid interpretations in many human studies with regard to the mechanism of action(s) of caffeine and/or its metabolites. The rate of metabolism of caffeine and other xanthine drugs also varies greatly from one individual to another. Therefore, it is extremely important to develop accurate, reliable analytical methods to quantify caffeine and its metabolites in simple and complex matrixes. A simple method is described for the separation and characterization of caffeine and its major metabolites employing capillary electrophoresis (CE) coupled to ultraviolet-absorption and mass spectrometry (MS) detection. After optimization of the electrophoresis separation conditions, a reliable separation of caffeine and 11 of its major metabolites was achieved in 50 mM ammonium carbonate buffer, pH 11.0. The volatile aqueous electrolyte system used with a normal electroosmotic flow polarity also provided an optimal separation condition for the characterization of the analytes by MS. The CE method achieved baseline resolution for all 12 compounds in less than 30 min. The CE-MS method is suitable for use as a routine procedure for the rapid separation and characterization of caffeine and its metabolites. The usefulness of this method was demonstrated by the extraction, separation, and identification of caffeine and its 11 metabolites from normal urine samples. The urine specimens were first acidified to obtain optimum binding efficiency to the sorbents of the off-line, solid-phase extraction procedure employed here, and an acidified eluent solvent was employed for the desorption step to maximize the recovery of the bound analytes.     

Interactions between poly(vinyl chloride) stabilised with epoxidised sunflower oil and food simulants

Commercial sunflower oil was epoxidised and used as organic co-stabiliser for rigid and plasticised poly(vinyl chloride) (PVC) containing Zn and Ca stearates as primary stabilisers and stearic acid as lubricant. For applications in the packaging of foodstuffs, migration testing must be performed. The detection and the quantification of contaminants migrating from the polymer into the food simulants are essential for the safety assessment of food contact plastic packaging materials. For that purpose, two food simulants were used: olive oil and 15% (v/v) aqueous ethanol. These represent fatty and moist food and beverages, respectively. The test conditions were 12 days at 40 °C. Circular samples of rigid PVC and PVC plasticised with dioctyl phthalate were immersed in a known volume of food simulant. A circular sample and 10 ml of food simulant were taken off every day to be analysed. Each sample was wiped and weighed. The rate of variation of the mass was determined as a function of time. The evolution of the peroxide index of olive oil with time was analysed. The specific migrations of the present additives were investigated by using two analytical methods (atomic absorption spectrometry and Fourier transform infrared spectroscopy). The influence of various parameters such as the nature of food simulant, the presence or the absence of the plasticiser, the agitation and time of contact was considered.     

Packed-column hydrodynamic chromatography using 1-μm non-porous silica particles

150×3 mm I.D. columns, packed with 1-μm non-porous spherical silica particles, were used to separate soluble synthetic polymers by hydrodynamic chromatography. The columns exhibited a plate height of about 1.4 μm allowing very fast and efficient separations of polymers in the molecular mass range 10³−2·10⁶ g/mol. The migration behaviour of polymers could be well described by a simple theoretical model. The applicability of packed bed HDC for the fast separation of polymers was illustrated with separations of polystyrene and poly(methyl methacrylate) mixtures.     

多层长渡越时间轴向绝缘堆的闪络概率分析

 对基于J.C.Martin经验公式的多层长渡越时间轴向绝缘堆的闪络概率分析方法进行了分析与总结。在对比各种类似分析方法优劣的基础上，提出了一种较为成熟的针对多层长渡越时间轴向绝缘堆的闪络概率分析方法，为类似绝缘堆结构的设计提供了理论依据。同时，对其中的不确定因素和存在的问题也进行了较为详细的分析和讨论，为闪络概率分析方法的进一步完善奠定了基础。     

Correlation free-solution capillary electrophoresis migration times of small peptides with physicochemical properties

Summary A series of small peptides containing varying degree of charge and size was used to study the effects of physicochemical properties on migration in free-solution capillary electrophoresis (FSCE). A semiempirical relationship between migration time under acidic conditions and the square root of molecular weight divided by the quantity of the number of the positively ionizable groups has been established. The ionization of the carboxyl terminal group in the polypeptides is negligible under acidic conditions. The relationship developed from this work has been used for the prediction of migration parameters in free solution capillary electrophoresis.     

Weighted Ehrhart theory and orbifold cohomology

We introduce the notion of a weighted δ-vector of a lattice polytope. Although the definition is motivated by motivic integration, we study weighted δ-vectors from a combinatorial perspective. We present a version of Ehrhart Reciprocity and prove a change of variables formula. We deduce a new geometric interpretation of the coefficients of the Ehrhart δ-vector. More specifically, they are sums of dimensions of orbifold cohomology groups of a toric stack.     

Comparing Algorithms for Sorting with <Emphasis Type="Italic">t</Emphasis> Stacks in Series

We show that the left-greedy algorithm is a better algorithm than the right-greedy algorithm for sorting permutations using t stacks in series when t > 1. We also supply a method for constructing some permutations that can be sorted by t stacks in series and from this get a lower bound on the number of permutations of length n that are sortable by t stacks in series. Finally we show that the left-greedy algorithm is neither optimal nor defines a closed class of permutations for t > 2.AMS Subject Classification: 05A05, 68R05, 68W01.